Talks for the CRC Members in 2017

by Prof. Dr. Nicole Pamme

14.12.2017, 17:00 h, TF, Aquarium

Microfluidic ”lab on chip” devices offer fascinating opportunities for fundamental research as well as real life applications. Handling fluids in micrometer sized channels allows for spatial and temporal control over experimental conditions at the size scale of cells and biomolecules. In our research group, we are employing magnetic forces to manipulate objects such as cells, particles and droplets inside microfluidic chips for applications in life sciences which enable fast analysis or fast processing with small volumes and minimal user input.

by Prof. Dr. Stefan Jurga

09.11.2017, 17:00 h, TF, Aquarium

Although still in its infancy, the field of multimodal nanomaterials has shown great potential in emerging biomedical fields such as imaging (optical, MRI), theranostics (therapy and diagnosis), including drug and gene delivery. The development of theranostic nanoplatforms for simultaneous therapy and diagnosis particularly in cancer treatment is enabled by the use of multifunctional nanomaterials or combination of nanomaterials with different functionalities. In this talk, I will summarize several types of multi-functional nanomaterials, based on carbon nanomaterials [1], quantum dots [2,3], nanoparticles containing rare earth elements [4], magnetic iron oxide nanoparticles [5,6], and metallic nanoparticles [7-8]. Special attention will be paid to application of multifunctional magnetic nanoplatform in gene therapy of glioblastoma multiform. We demonstrate the application of magnetic nanoparticles coated with polyethylenimine for nano-mediated RNAi therapy of glioblastoma by downregulating Tenascin-C (TN-C). The obtained nanomaterials were characterized by FTIR,TEM,SQUID and Zeta-potential. The broad spectrum of test including WST, SRB, LIVE/DEAD assays were performed to evaluate the cytotoxicity of nanocomposites. Their efficiency in downregulation of TN-C was assessed by means of RT-PCR and internalization of nanocomposites was checked by confocal microscopy. Moreover, contrast properties of nanomaterial were investigated by MRI. In conclusion, our results indicate that synthesized nanoplatform can serve as theranostic tool for glioblastoma treatment.

References

[1] Baranowska-Korczyc A. et al. PEG–MWCNT/Fe hybrids as multi-modal contrast agents for MRI and optical imaging, RSC Adv 2016 (55) 49891-49902.

[2] Michalska M. et al. Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging, Acta Biomater 2016 (35) 293-304.

[3] Przysiecka Ł. et al., iRGD peptide as effective transporter of CuInZnxS2+x quantum dots into human cancer cells, Colloids Surf B Biointerfaces 146 (2016) 9-18.

[4] N. Babayevska et al. Functionalized multimodal ZnO@Gd2O3 nanosystems to use as perspective contrast agent for MRI, Appl Surf Sci 2017 (404) 129-137.

[5] Grześkowiak B.F. et al. Nanomagnetic activation as a way to control the efficacy of nucelic acid delivery, Pharm Res 2015 (32) 103-121.

[6] Mrówczyński R. et al. Assessment of polydopamine coated magnetic nanoparticles in doxorubicin delivery, RSC Adv 2016 (6) 5936-5943.

[7] Woźniak-Budych M. et al. Green synthesis of rifampicin-loaded copper nanoparticles with enhanced antimicrobial activity , J Mater Sci: Mater Med 2017 (28) 42-45.

[8] Woźniak A. et al. Cytotoxicity and imaging studies of β-NaGdF4:Yb3+Er3+@ PEG-Mo nanorods, RSC Adv 2016 (6) 95633-95643.

by Dr. Myriam Pannetier-Lecoeur

12.10.2017, 17:00 h, TF, Aquarium

Currents circulating in excitable cells like neurons or nerve fibers may be measured by the radiated magnetic field. At the organ level, these magnetic fields can be detected by non-invasive experiments using highly sensitive magnetometers such as SQUIDS, atomic magnetometers or mixed sensors, the latter using spin electronics. To understand the genesis of the signals obtained in brain areas, it is relevant to investigate the fields generated at the level of one or few cells. This requires small and sensitive field sensors, operating at physiological temperatures, which has long been out of reach from existing technologies. Spin electronics, based on thin film magnetic properties, explores the variation of conduction electron transport as a function of the state of their spin. It is thus possible to modify the resistance of an element as a function of the magnetic field of its environment. This property has been widely exploited in hard disk drive heads, but also opens up the possibility of manufacturing very sensitive and miniaturizable magnetic sensors. Spin electronics-based magnetic sensors are micron-size devices reaching sub-nanotesla field range on a wide range of temperature, including physiological temperature. We have designed and fabricated magnetic sensors called magnetrodes, as a magnetic equivalent of electrodes, to probe locally the information transmission of excitable cells. These probes contain one or several GMR elements in embodiment compatible to recordings in contact with tissues or within tissues. Two types of sensors have been evaluated on living tissues; a planar probe to investigate the Action Potential propagation in in vitro preparation of muscle cells, which have demonstrated the first local biomagnetic recordings with GMR sensors, and a sharp probe for in vivo recordings of cortical activity. In this talk I will present how sensors based on spin electronics can address biomagnetic signal recordings at the organ level and at local scale. In particular I will discuss the first in vivo experiments performed, which have paved a new way to a local description of electrical activity, without direct contact to the cell and which allow accessing not only the amplitude of the activity but also its direction of propagation, at any depth within the tissues.

by Prof. Dr. med. Wilhelm Schulte-Mattler

14.09.2017, 17:00 h, TF, Aquarium

 

German title

Physiologie peripherer Nerven aus Sicht der Signalverarbeitung

 

Abstract

To transmit information, peripheral nerve fibers locally change their electrical membrane properties. The changed regions move along the fibers causing traveling electrical fields, causing changes in voltage over time that depend both on where the voltage is recorded and on the nerve’s properties. Things are complicated by the nerves being composed of many thousands of fibers.

A simple model that explains these voltage changes, namely the signals that are recorded from actively transmitting nerves, will be presented. These signals provide information about the nerve’s function. Both, the influence of the recording conditions and the influence of various nerve disorders on the recorded waveforms will be presented. The usefulness of simple measures, such as amplitude and duration, is established. More advanced signal analysis indeed provides more information about peripheral nerve disorders.

 

Short biography

Wilhelm Schulte-Mattler studied Mathematics and Physics, followed by Medicine. He graduated at the University of Würzburg in 1988. His thesis was on Quantification of recruitment in needle-EMG. He specialized in Neurology in 1993. After heading Clinical Neurophysiology in the Dept. of Neurology, University of Halle-Wittenberg; since the year 2000, he is head of Clinical Neurophysiology in the Dept. of Neurology, University of Regensburg. A significant part of his work is on waveform analysis in clinical neurophysiology, particularly in electromyography and in electroneurography.

Contact

sfb1261@tf.uni-kiel.de

Chairman:

Prof. Dr. Eckhard Quandt

Kiel University
Institute for Materials Science

 

Interner Server

 

CAU

Christian-Albrechts-Universität zu Kiel (CAU)

Christ.-Albrechts-Platz 4
D-24118 Kiel

UKSH

University Hospital Schleswig-Holstein, Campus Kiel (UKSH)

Arnold-Heller-Straße 3
D-24105 Kiel

ISIT

Fraunhofer Institute for Silicon Technology, Itzehoe (ISIT)

Fraunhoferstrasse 1
D-25524 Itzehoe  

IPN

IPN - Leibniz-Institut für die Pädagogik der Naturwissenschaften und Mathematik an der Universität Kiel

Olshausenstraße 62 
D-24118 Kiel