by Prof. Dr. Stefan Jurga
09.11.2017, 17:00 h, TF, Aquarium
Although still in its infancy, the field of multimodal nanomaterials has shown great potential in emerging biomedical fields such as imaging (optical, MRI), theranostics (therapy and diagnosis), including drug and gene delivery. The development of theranostic nanoplatforms for simultaneous therapy and diagnosis particularly in cancer treatment is enabled by the use of multifunctional nanomaterials or combination of nanomaterials with different functionalities. In this talk, I will summarize several types of multi-functional nanomaterials, based on carbon nanomaterials , quantum dots [2,3], nanoparticles containing rare earth elements , magnetic iron oxide nanoparticles [5,6], and metallic nanoparticles [7-8]. Special attention will be paid to application of multifunctional magnetic nanoplatform in gene therapy of glioblastoma multiform. We demonstrate the application of magnetic nanoparticles coated with polyethylenimine for nano-mediated RNAi therapy of glioblastoma by downregulating Tenascin-C (TN-C). The obtained nanomaterials were characterized by FTIR,TEM,SQUID and Zeta-potential. The broad spectrum of test including WST, SRB, LIVE/DEAD assays were performed to evaluate the cytotoxicity of nanocomposites. Their efficiency in downregulation of TN-C was assessed by means of RT-PCR and internalization of nanocomposites was checked by confocal microscopy. Moreover, contrast properties of nanomaterial were investigated by MRI. In conclusion, our results indicate that synthesized nanoplatform can serve as theranostic tool for glioblastoma treatment.
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